The research developed is part of the proposal of new methods using luminescent techniques for the development of rapid response analytical platforms. These platforms constitute dynamic and miniaturized analytical (bio)sensor systems combined with the incorporation of hybrid nanomaterials in the (bio)active zone. Two lines have been developed within the proposed platforms: (i) based on the use of flat sensors and (ii) microfluidic systems. In both cases, improvements in the physicochemical and structural properties of nanomaterials are explored, proposing hybrid structures with different levels of functionalization, to favor the sensitivity and selectivity of the proposed methods. Among the synthesized nanomaterials are: silica, metallic (Ag, Au) and magnetic nanoparticles; hybrid liposomes in which nanomaterials or other molecules are encapsulated; or nanomaterials close to the sub-nanometric scale, such as metallic nanoclusters (MeNCs), synthesized within biomolecular structures (proteins and oligonucleotides), or carbon dots (CDs), luminescent carbon nanomaterials. The focus is also on the spectroscopic, microscopic and structural characterization of the synthesized nanomaterials. Regarding the design of the analytical platforms, auxiliary microdevices will be integrated that will facilitate the development of the analytical process at micro and nanometric scales. The object of this research extends to remote monitoring applications in environmental, agrifood and clinical-toxicological analysis.