Copper acquisition is essential for plant colonization and virulence in a root-infecting vascular wilt fungus

Palos-Fernández R, Aguilar-Pontes MV, Puebla-Planas G, Berger H, Studt-Reinhold L, Strauss J, Di Pietro A, López-Berges MS.

PloS Pathog 20(11):e1012671. Link

Evolution of the pathogenic mold Aspergillus fumigatus on high copper levels identifies novel resistance genes

Handelman M, Meir Z, Shadkchan Y, Kandil AA, Amano O, Mariscal M, López-Berges MS, Osherow N.

mSphere 9:e00253-24 Link

Molecular dialogue during host manupulation by the vascular wilt fungus Fusarium oxysporum.

Srivastava V, Patra K, Pai H, Aguilar-Pontes MV, Berasategui A, Kamble A, Di Pietro A, Redkar A. 
Annu Rev Plant Pathol 62. Link

Agrios' Plant Pathology (Sixth edition)

Oliver, R (ed), Hückelhoven R, del Ponte E, Di Pietro A. (co-eds) Academic Press Inc. ISBN 978-0-12-822429-8 Link

Constitutive activation of TORC1 signalling attenuates virulence in the cross-kingdom fungal pathogen Fusarium oxysporum.
Navarro-Velasco GY, Di Pietro A, López-Berges MS. 
Mol Plant Pathol 24:289-301. Link

Cytosolic pH controls fungal MAPK signaling and pathogenicity
Fernandes TR, Mariscal M, Serrano A, Segorbe D, Fernández-Acero T, Martín H, Turrà D, Di Pietro A
mBio 00:e00285-23. Link

Role of pH in the control of fungal MAPK signalling and pathogenicity
Mariscal M, Fernandes TR, Di Pietro A
Series: The Mycota, vol 5. Scott, B., Mesarich, C. (eds) Plant Relationships. Springer, Cham. ISBN 978-3-031-16503-0 Link

Fusarium oxysporum casein kinase 1, a negative regulator of the plasma membrane H⁺-ATPase Pma1, is required for development and pathogenicity.
Mariscal M, Miguel-Rojas C, Hera C, Fernandes TR, Di Pietro A.
J Fungi 8:1300. Link

The Gal4-type transcription factor Pro1 integrates inputs from two different MAPK cascades to regulate development in the fungal pathogen Fusarium oxysporum.
Palos-Fernández R, Turrà D, Di Pietro A.
J Fungi 8:1242. Link

Conserved secreted effectors contribute to endophytic growth and multi-host plant compatibility in a vascular wilt fungus.
Redkar A, Sabale M, Schudoma C, Zechmann B, Gupta YK, López-Berges MS, Venturini G, Gimenez-Ibanez S, Turrà D, Solano R, Di Pietro A.
The Plant Cell 34:3214-3232. Link

Determinants of endophytic and pathogenic lifestyle in root colonizing fungi.
Redkar A, Sabale M, Zuccaro A, Di Pietro A.
Curr Opin Plant Biol 67:102226. Link

Impairment of the cellulose degradation machinery enhances Fusarium oxysporum virulence but limits its reproductive fitness.
Gámez-Arjona FM, Vitale S, Voxeur A, Dora S, Müller S, Sancho-Andrés G, Montesinos JC, Di Pietro A, Sánchez-Rodríguez C.
Sci Adv 8:eabl9734. Link

Localisation of phosphoinositides in the grass endophyte Epichloë festucae and genetic and functional analysis of key components of their biosynthetic pathway in E. festucae symbiosis and Fusarium oxysporum pathogenesis.
Hassing B, Candy A, Eaton CJ, Fernandes TR, Mesarich CH, Di Pietro A, Scott B.
Fungal Genet Biol 159:103669. Link

Marchantia polymorpha model reveals conserved infection mechanisms in the vascular wilt fungal pathogen Fusarium oxysporum.
Redkar A, Gimenez Ibanez S, Sabale M, Zechmann B, Solano R, Di Pietro A.
New Phytol 234:227-241. Link

In vivo monitoring of cytosolic pH using the ratiometric pH sensor pHluorin.
Fernandes TR, Serrano A, Di Pietro A.
Methods Mol Biol 2391:99-107. Link

Quantification and isolation of spontaneous colony growth variants.
Díaz CL, Gómez-Gil L, Pérez-Nadales E, Velasco GN, Di Pietro A.
Methods Mol Biol 2391:55-62. Link

Chemotropic assay for testing fungal response to strigolactones and strigolactone-like compounds.

Pineda-Martos, R, Di Pietro A, Turrà D. 

Methods Mol Biol 2309:105-111. Link

A 'hydrolase switch' for vascular specialization in plant pathogenic bacteria.
Redkar A, Sabale M, Di Pietro A.
Trends Plant Sci 26:427-429. Link

Efinder: a bioinformatics pipeline for detecting new transposable element insertion events in next-generation sequencing data.
Sohrab V, López-Díaz C, Di Pietro A, Ma LJ, Ayhan DH.
Genes (Basel) 12:224. Link

Dual-specificity protein phosphatase Msg5 controls cell wall integrity and virulence in Fusarium oxysporum.
Fernandes TR, Sánchez Salvador E, Tapia ÁG, Di Pietro A.
Fungal Genet Biol 146:103486. Link

A bacterial endophyte exploits chemotropism of a fungal pathogen for plant colonization.
Palmieri D, Vitale S, Lima G, Di Pietro A, Turrà D.
Nat Commun 11:5264. Link

Lineage-specific genes and cryptic sex: parallels and differences between arbuscular mycorrhizal fungi and fungal pathogens.
Reinhardt D, Roux C, Corradi N, Di Pietro A.
Trends Plant Sci S1360-1385(20)30273-9. Link

Chemotropism assays for plant symbiosis and mycoparasitism related compound screening in Trichoderma atroviride.
Moreno-Ruiz D, Lichius A, Turrà D, Di Pietro A, Zeilinger S.
Front Microbiol 11:601251. Link

Structure of fungal α mating pheromone in membrane mimetics suggests a possible role for regulation at the water-membrane interface.
Partida-Hanon A, Maestro-López M, Vitale S, Turrà D, Di Pietro A, Martínez-del Pozo A, Bruix M.
Front Microbiol 11:1090. Link

The genome of opportunistic fungal pathogen Fusarium oxysporum carries a unique set of lineage-specific chromosomes.
Zhang Y, Yang H, Turra D, Zhou S, Ayhan DH, DeIulio GA, Guo L, Broz K, Wiederhold N, Coleman JJ, Donnell KO, Youngster I, McAdam AJ, Savinov S, Shea T, Young S, Zeng Q, Rep M, Pearlman E, Schwartz DC, Di Pietro A, Kistler HC, Ma LJ.
Commun Biol 3:50. Link

ZafA-mediated regulation of zinc homeostasis is required for virulence in the plant pathogen Fusarium oxysporum.
López-Berges MS.
Mol Plant Pathol 21:244-249. Link

The phosphatase Ptc6 is involved in virulence and MAPK signalling in Fusarium oxysporum.
Nunez-Rodriguez JC, Ruiz-Roldán C, Lemos P, Membrives S, Hera C.
Mol Plant Pathol 21:206-217. Link

Expansion microscopy for cell biology analysis in fungi.
Götz R, Panzer S, Trinks N, Eilts J, Wagener J, Turrà D, Di Pietro A, Sauer M, Terpitz U.
Front Microbiol 11:574. Link

Rapid evolution in plant-microbe interactions - a molecular genomics perspective. Frantzeskakis L, Di Pietro A, Rep M, Schirawski J, Wu CH, Panstruga R.
New Phytol 225:1134-1142. Link

Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum.
Lorenzo-Gutiérrez D, Gómez-Gil L, Guarro J, Roncero MIG, Capilla J, López-Fernández L.
Virulence 11:1108-1121. Link

Autocrine pheromone signalling regulates community behaviour in the fungal pathogen Fusarium oxysporum.
Vitale S, Di Pietro A, Turrà D.
Nat Microbiol 4:1443-1449. Link

NADPH oxidase regulates chemotropic growth of the fungal pathogen Fusarium oxysporum towards the host plant.
Nordzieke DE, Fernandes TR, El Ghalid M, Turrà D, Di Pietro A.
New Phytol 224:1600-1612. Link

The role of volatile organic compounds and rhizosphere competence in mode of action of the non-pathogenic Fusarium oxysporum FO12 toward Verticillium wilt.
Mulero-Aparicio A, Cernava T, Turrà D, Schaefer A, Di Pietro A, López-Escudero FJ, Trapero A, Berg G.
Front Microbiol 10:1808. Link

A conserved microbial motif 'traps' protease activation in host immunity.
Sabale M, Di Pietro A, Redkar A.
Trends Plant Sci 24:665-667. Link

Role of the Fusarium oxysporum metallothionein Mt1 in resistance to metal toxicity and virulence.
Lorenzo-Gutiérrez D, Gómez-Gil L, Guarro J, Roncero MIG, Fernández-Bravo A, Capilla J, López-Fernández L.
Metallomics 11:1230-1240. Link

How alkalinization drives fungal pathogenicity.
Fernandes TR, Segorbe D, Prusky D, Di Pietro A.
PLoS Pathog 13:e1006621. Link

Fungal pathogenesis: Combatting the oxidative burst.
Di Pietro A, Talbot NJ.
Nat Microbiol: 2:17095. Link

Regulatory mechanisms of a highly pectinolytic mutant of Penicillium occitanis and functional analysis of a candidate gene in the plant pathogen Fusarium oxysporum.
Bravo-Ruiz G, Sassi AH, Marcet-Houben M, Di Pietro A, Gargouri A, Gabaldon T, Roncero MIG.
Front Microbiol 8:1627. Link

Stress Adaptation.
Brown AJP, Cowen LE, Di Pietro A, Quinn J.
Microbiol Spectr. 5: doi: 10.1128/microbiolspec.FUNK-0048-2016. Link

Vacuolar control of subcellular cation distribution is a key parameter in the adaptation of Debaryomyces hansenii to high salt concentrations.
Herrera R, Salazar A, Ramos-Moreno L, Ruiz-Roldan C, Ramos J.
Fungal Genet Biol 100:52-60. Link

Structure-activity relationship of α mating pheromone from the fungal pathogen Fusarium oxysporum.
Vitale S, Partida-Hanon A, Serrano S, Martínez-Del-Pozo Á, Di Pietro A, Turrà D, Bruix M.
J Biol Chem 292:3591-3602. Link

Three Fusarium oxysporum mitogen-activated protein kinases (MAPKs) have distinct and complementary roles in stress adaptation and cross-kingdom pathogenicity.
Segorbe D, Di Pietro A, Pérez-Nadales E, Turrà D.
Mol Plant Pathol 18:912-924. Link

A fungal pathogen secretes plant alkalinizing peptides to increase infection.
Masachis S, Segorbe D, Turrà D, Leon-Ruiz M, Fürst U, El Ghalid M, Leonard G, López-Berges, MS, Richards TA, Felix G, Di Pietro A.
Nat Microbiol 1:16043. Link

Hyphal chemotropism in fungal pathogenicity.
Turrà D, Nordzieke D, Vitale S, El Ghalid M, Di Pietro A.
Semin Cell Dev Biol 57:69-75. Link

Fusaric acid contributes to virulence of Fusarium oxysporum on plant and mammalian hosts.
López-Díaz C, Rahjoo V, Sulyok M, Ghionna V, Martín-Vicente A, Capilla J, Di Pietro A, López-Berges MS.
Mol Plant Pathol 19:440-453. Link

Adapt your shuttling proteins for virulence: a lesson from the corn smut fungus Ustilago maydis.
Redkar A, Di Pietro A.
New Phytol 220:353-356. Link

Role of the phosphatase Ptc1 in stress responses mediated by CWI and HOG pathways in Fusarium oxysporum.
Lemos P, Ruiz-Roldán C, Hera C.
Fungal Genet Biol 118:10-20. Link

Improved assembly of reference genome Fusarium oxysporum f. sp. lycopersici strain Fol4287.
Ayhan DH, López-Díaz C, Di Pietro A, Ma LJ.
Microbiol Resour Announc 7:e00910-18. Link

Live-cell imaging of conidial anastomosis tube fusion during colony initiation in Fusarium oxysporum.
Kurian SM, Di Pietro A, Read ND.
PLoS One 13:e0195634. Link

Nitrate assimilation pathway (NAP): role of structural (nit) and transporter (ntr1) genes in Fusarium oxysporum f.sp. lycopersici growth and pathogenicity.
Gomez-Gil L, Camara Almiron J, Rodriguez Carrillo PL, Olivares Medina CN, Bravo Ruiz G, Romo Rodriguez P, Corrales Escobosa AR, Gutierrez Corona F, Roncero MI.
Curr Genet 64:493-507. Link

Fungal pathogen uses sex pheromone receptor for chemotropic sensing of host plant signals.
Turrà D, El Ghalid M, Rossi F, Di Pietro A.
Nature 527:521-524. Link

A conserved co-chaperone is required for virulence in fungal plant pathogens.
Lo Presti L, López Díaz C, Turrà D, Di Pietro A, Hampel M, Heimel K, Kahmann R.
New Phytol 209:1135-1148. Link

Data quality aware analysis of differential expression in RNA-seq with NOISeq R/Bioc package.
Tarazona S, Furio-Tari P, Turrà D, Di Pietro A, Nueda MJ, Ferrer A, Conesa A.
Nucleic Acids Res 43:e140. Link

Chemotropic sensing in fungus-plant interactions.
Turrà D, Di Pietro A.
Curr Opin Plant Biol 26:135-140. Link

Comparative proteomic analyses reveal that Gnt2-mediated N-glycosylation affects cell wall glycans and protein content in Fusarium oxysporum.
Lopez-Fernandez L, Roncero MI, Prieto A, Ruiz-Roldan C.
J Proteomics 128:189-202. Link

Combined action of the major secreted exo- and endopolygalacturonases is required for full virulence of Fusarium oxysporum.
Bravo Ruiz G, Di Pietro A, Roncero MIG.
Mol Plant Pathol 17:339-353. Link

Autophagy contributes to regulation of nuclear dynamics during vegetative growth and hyphal fusion in Fusarium oxysporum.
Corral-Ramos C, Roca MG, Di Pietro A, Roncero MIG, Ruiz-Roldán C.
Autophagy 11:131-144. Link

The transcription factor Con7-1 is a master regulator of morphogenesis and virulence in Fusarium oxysporum.
Ruiz-Roldán C, Pareja-Jaime Y, González-Reyes JA, Roncero MI.
Mol Plant Microbe Interact 28:55-68. Link

Protein kinases in plant pathogenic fungi: conserved regulators of infection.
Turrà D, Segorbe D, Di Pietro A.
Annu Rev Phytopathol 52: 267-288. Link

The transmembrane protein Sho1 cooperates with the mucin Msb2 to regulate invasive growth and plant infection in Fusarium oxysporum.
Pérez-Nadales E, Di Pietro A.
Mol Plant Pathol 16:593-603. Link

The Secreted Peptide PIP1 Amplifies Immunity through Receptor-Like Kinase 7.
Hou S, Wang X, Chen D, Yang X, Wang M, Turrà D, Di Pietro A, Zhang W.
PLoS Pathog 10:e1004331. Link

Fungal model systems and the elucidation of pathogenicity determinants.
Perez-Nadales E, Almeida Nogueira MF, Baldin C, Castanheira S, El Ghalid M, Grund E, Lengeler K, Marchegiani E, Mehrotra PV, Moretti M, Naik V, Oses-Ruiz M, Oskarsson T, Schäfer K, Wasserstrom L, Brakhage AA, Gow NA, Kahmann R, Lebrun MH, Perez-Martin J, Di Pietro A, Talbot NJ, Toquin V, Walther A, Wendland J.
Fungal Genet Biol 70C:42-67. Link

Hyphal growth of phagocytosed Fusarium oxysporum causes cell lysis and death of murine macrophages.
Schäfer K, Bain JM, Di Pietro A, Gow NAR, Erwig LP.
PLoS One 9:e101999. Link

Murine model for Fusarium oxysporum invasive fusariosis reveals organ specific structures for dissemination and long-term persistence.
Schäfer K, Di Pietro A, Gow NAR, MacCallum D.
PLoS One 9:e89920. Link

Combinatorial function of Velvet and AreA in transcriptional regulation of nitrate utilization and secondary metabolism.
López-Berges M, Schäfer K, Hera C, Di Pietro A.
Fungal Genet Biol 62:78-84. Link

Targeting iron acquisition blocks infection with the fungal pathogens Aspergillus fumigatus and Fusarium oxysporum.
Leal SM Jr, Roy S, Vareechon C, Carrion SD, Clark H, Lopez-Berges MS, Di Pietro A, Schrettl M, Beckmann N, Redl B, Haas H, Pearlman E.
PLOS Pathog 9:e1003436. Link

The CAP protein superfamily: function in sterol export and fungal virulence.
Schneiter R, Di Pietro A.
Biomol Concepts 4:519-526. Link

The Fusarium oxysporum gnt2, encoding a putative N-acetylglucosamine transferase, is involved in cell wall architecture and virulence.
López-Fernández L, Ruiz-Roldán C, Pareja-Jaime Y, Prieto A, Khraiwesh H, Roncero MI.
PLoS One 8:e84690. Link

Iron competition in fungus-plant interactions: The battle takes place in the rhizosphere.
López-Berges MS, Turrà D, Capilla J, Schafferer L, Matthijs S, Jöchl C, Cornelis P, Guarro J, Haas H, Di Pietro A.
Plant Signal Behav 8:e23012. Link

The velvet complex governs mycotoxin production and virulence of Fusarium oxysporum on plant and mammalian hosts.
López-Berges MS, Hera C, Sulyok M, Schäfer K, Capilla J, Guarro J, Di Pietro A.
Mol Microbiol 87:49-65. Link

Lipolytic system of the tomato pathogen Fusarium oxysporum f. sp. lycopersici.
Bravo-Ruiz G, Ruiz-Roldán C, Roncero MI.
Mol Plant Microbe Interact 26:1054-1067. Link

HapX-mediated iron homeostasis is essential for rhizosphere competence and virulence of the soilborne pathogen Fusarium oxysporum.
López-Berges MS, Capilla J, Turrà D, Schafferer L, Matthijs S, Jöchl C, Cornelis P, Guarro J, Haas H, Di Pietro A.
Plant Cell 24:3805-3822. Link

A PR-1-like protein of Fusarium oxysporum functions in virulence on mammalian hosts.
Prados-Rosales RC, Roldan-Rodriguez R, Serena C, Lopez-Berges MS, Guarro J, Martinez-Del-Pozo A, Di Pietro A.
J Biol Chem 287:21970-21979. Link

The Top 10 fungal pathogens in molecular plant pathology.
Dean R, VAN Kan JA, Pretorius ZA, Hammond-Kosack KE, DI Pietro A, Spanu PD, Rudd JJ, Dickman M, Kahmann R, Ellis J, Foster GD.
Mol Plant Pathol 13:414-430. Link

Morphogenesis and pathogenicity in fungi.
Pérez-Martín J, Di Pietro A (eds.)
Series: Topics in Current Genetics, Vol 22, Springer Verlag, Heidelberg, Berlin. 286 pp. ISBN: 978-3-642-22915-2. Link

The membrane mucin Msb2 regulates invasive growth and plant infection in Fusarium oxysporum.
Pérez-Nadales E, Di Pietro A.
Plant Cell 23:1171-1185. Link

Galleria mellonella as model host for the trans-kingdom pathogen Fusarium oxysporum.
Navarro-Velasco GY, Prados-Rosales RC, Ortíz-Urquiza A, Quesada-Moraga E, Di Pietro A.
Fungal Genet Biol 48:1124-1129. Link

Role of new bacterial surfactins in the antifungal interaction between Bacillus amyloliquefaciens and Fusarium oxysporum.
Vitullo D, Di Pietro A, Romano A, Lanzotti V, Lima G.
Plant Pathol 61:689-699. Link

Antifungal lipopeptides from Bacillus amyloliquefaciens strain BO7.
Romano A, Vitullo D, Di Pietro A, Lima G, Lanzotti V.
J Nat Prod 74:145-151. Link

A nitrogen response pathway regulates virulence functions in Fusarium oxysporum via the protein kinase TOR and the bZIP protein MeaB.
López-Berges MS, Rispail N, Prados-Rosales RC, Di Pietro A.
Plant Cell 22: 2459-2475. https://doi.org/10.1105/tpc.110.075937

Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium oxysporum.
Ma LJ, van der Does C, Borkovich KA, Coleman JJ, Daboussi MJ, Di Pietro A, Dufresne M, Freitag M, Grabherr M, Henrissat B, Houterman PM, Kang S, Shim WB, Woloshuk C, Xie X, Xu JR, Antonxiw J, Baker SE, Bluhm BH, Breakspear A, Brown DW, Butchko RAE, Chapman S, Coulson R, Coutinho PM, Danchin EGJ, Diener A, Gale LR, Gardiner DM, Goff S, Hammond-Kosack KE, Hilburn K, Houterman PM, Hua-Van A, Jonkers W, Kazan K, Kodira CD, Koehrsen M, Kumar L, Lee YH, Li L, Manners JM, Miranda-Saavedra D, Mukherjee M, ParkG, Park J, Park SY, Proctor RH, Regev A, Ruiz-Roldan MC, Sain D, Sakthikumar S, SykesS, Schwartz DC, Turgeon BG, Wapinski I, Yoder O, Young S, Zeng Q, Zhou S, Galagan J, Cuomo CA, Kistler HC, Rep M.
Nature 464: 367-373. Link

The molecular pathogenicity of Fusarium keratitis: a fungal transcriptional regulator promotes hyphal penetration of the cornea.
Hua X, Yuan X, Di Pietro A, Wilhelmus KR.
Cornea 29:1440-1444. Link

Nuclear dynamics during germination, conidiation and hyphal fusion of Fusarium oxysporum.
Ruiz-Roldán MC, Köhli M, Roncero MIG, Philippsen P, Di Pietro A, Espeso E.
Eukaryot Cell 9: 1216 -1224. Link

The homeodomain transcription factor Ste12: Connecting fungal MAPK signalling to plant pathogenicity.
Rispail N, Di Pietro A.
Commun Integr Biol 3:327-332. Link

A nitrogen response pathway regulates virulence in plant pathogenic fungi: role of TOR and the bZIP protein MeaB.
López-Berges MS, Rispail N, Prados-Rosales RC, Di Pietro A.
Plant Signal Behav 5:1623-1625. Link

The two-component histidine kinase Fhk1 controls stress adaptation and virulence of Fusarium oxysporum.
Rispail N, Di Pietro A.
Mol Plant Pathol 11: 395-407. Link